Skip to Content
Methods in enzymology. v.234, Oxygen radicals in biological systems. Part D

Methods in enzymology. v.234, Oxygen radicals in biological systems. Part D

Packer, Lester

Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 233 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. It includes techniques and assays of the activity of biothiols in soluble systems, their interaction with lipids and proteins, and the various new thiol enzymes, such as thioltransferases, being elucidated. Antioxidant reactions and gene regulation are also included

Book. English.
Published San Diego: Academic Press, c1994

Available at O'Reilly Library.

This item is not reservable because:

  • There are no reservable copies for this title. Please contact a member of library staff for further information.
  • O'Reilly Library – One available in Main Collection 572.7

    Barcode Shelfmark Loan type Status
    940827123 Main Collection 572.7 3 Weeks Available


Statement of responsibility: edited by Lester Packer. Part D
ISBN: 0121821358, 9780121821357
Note: Includes bibliographical references and indexes.
Physical Description: xxxiii, 704 p : ill. ; 24 cm.
Subject: Radicals (Chemistry); Active oxygen.
Added Entry: Oxygen radicals in biological systems


  1. Oxidative Damage to DNA and DNA Repair
  2. M. Dizdaroglu, Chemical Determination of Oxidative DNA Damage by Gas Chromatography-Mass Spectrometry.
  3. M.K. Shigenaga, E.N. Aboujaoude, Q. Chen, and B.N. Ames, Assays of the Oxidative DNA Damage Biomarker 8-Oxo-2-deoxyguanosine and 8-Oxoguanine in Nuclear DNA and Biological Fluids by High-Performance Liquid Chromatography with Electrochemical Detection.
  4. K.K. Hamilton, K. Lee, and P.W. Doetsch, Detection and Characterization of Eukaryotic Enzymes That Recognize Oxidative DNA Damage.
  5. W.A. Schulz, M.S.W. Obendorf, and H. Sies, Localization of Strand Breaks in Plasmid DNA Treated with Reactive Oxygen Species.
  6. Y. Luo, E.S. Henle, R. Chatopadhyaya, R. Jin, and S. Linn, Detecting DNA Damage Caused by Iron and Hydrogen Peroxide.
  7. P.K. Wong and R.A. Floyd, Photochemical Synthesis of 8-Hydroxyguanine Nucleosides.
  8. M.J. Burkitt, Copper (DNA Adducts).
  9. J. Cadet. J.-L. Ravanat, G.W. Buchko, H.C. Yeo, and B.N. Ames, Singlet Oxygen DNA Damage: Chromatographic and Mass Spectrometric Analysis of Damage Products.
  10. C. Baumstark-Khan, Alkaline Elution versus Fluorescence Analysis of DNA Unwinding.
  11. L. Augeri, K.K. Hamilton, A.M. Martin, P. Yohannes, and P.W. Doetsch, Purification and Properties of Yeast Redoxyendonuclease.
  12. C.F.M. Menck, Shuttle Vector between Prokaryotes and Eukaryotes for Assaying Singlet Oxygen-Induced DNA Damage and Mutagenicity.
  13. B. Epe and J. Hegler, Oxidative DNA Damage: Endonuclease Fingerprinting.
  14. Assay of Stress Genes/Proteins, Effects of Reactive Oxygen Species and Antioxidants on Cell Regulatory Processes
  15. W. Drige, S. Mihm, M. Bockstette, and S. Roth, Effect of Reactive Oxygen Intermediates and Antioxidants on Proliferation and Function of T Lymphocytes.
  16. R. Schreck and P.A. Baeuerle, Assessing Oxygen Radicals as Mediators in Activation of Inducible Eukaryotic Transcription Factor NF-(B.
  17. S. Xanthoudakis and T. Curran, Analysis of c-Fos and c-Jun Redox-Dependent DNA Binding Activity.
  18. D.R. Crawford, C.A. Edbauer-Nechaman, C.V. Lowry, S.L. Salmon, Y.K. Kim, J.M.S. Davies, and K.J.A. Davies, Assessing Gene Expression during Oxidative Stress.
  19. G. Storz and S. Altuvia, OxyR Regulon.
  20. R.M. Tyrrell and S. Basu-Modak, Transient Enhancement of Heme Oxygenase 1 mRNA Accumulation: A Marker of Oxidative Stress to Eukaryotic Cells.
  21. J.S. Bertram and L.-X. Zhang, Assays for Regulation of Gap Junctional Communication and Connexin Expression by Carotenoids.
  22. G.H.W. Wong and D.V. Goeddel, One-Day Northern Blotting for Detection of mRNA: NDGA Inhibits the Induction of Mn-SOD mRNA by Agonists of Type 1 Receptor.
  23. C.E. Cross and B. Halliwell, Evaluation of Biomolecular Damage by Ozone.
  24. B.K. Tarkington, T.R. Duvall, and J.A. Last, Ozone Exposure of Cultured Cells and Tissues.
  25. Antioxidant Identification, Characterization, and Assay: A. Total Antioxidant Status
  26. P.A. Motchnik, B. Frei, and B.N. Ames, Measurementof Antioxidants in Human Blood Plasma.
  27. C. Rice-Evans and N.J. Miller, Total Antioxidant Status in Plasma and Body Fluids.
  28. B. Natural Source Antioxidants: Vitamin E
  29. W. Schaep and R. Rettenmaier, Analysis of Vitamin E Homologs in Plasma and Tissue: High-Performance Liquid Chromatography.
  30. G. Riss, A.W. Kormann, E. Glinz, W. Walther, and U.B. Ranalder, Separation of the Eight Stereoisomers of all-rac-(-Tocopherol from Tissues and Plasma: Chiral Phase High-Performance Liquid Chromatography and Capillary Gas Chromatography.
  31. D.C. Liebler, Tocopherone and Epoxytocopherone Products of Vitamin E Oxidation.
  32. V.E. Kagan and L. Packer, Light-Induced Generation of Vitamin E Radicals: Assessing Vitamin E Regeneration.
  33. M. Yamaoka and K. Komiyama, Antioxidative Activity of Tocotrienol in Heterogeneous System: Indication of Restriction within Membrane by Fluorescence Measurement.
  34. G.T. Vatassery, Determination of Tocopherols and Tocopherolquinone in Human Red Blood Cell and Platelet Samples.
  35. Vitamin C
  36. G. Barja and A. Hernanz, Vitamin C, Dehydroascorbate, and Uric Acid in Tissues and Serum: High-Performance Liquid Chromatography.
  37. M. Inoue and K. Koyama, In Vivo Determination of Superoxide and Vitamin CRadicals Using Cytochrome c and Superoxide Dismutase Derivatives.
  38. Quinones
  39. V.E. Kagan, E.A. Serbinova, D.A. Stoyanovsky, S. Khwaja, and L. Packer, Assay of Ubiquinones and Ubiquinols as Antioxidants.
  40. E.A. Serbinova and L. Packer, Antioxidant Properties of (-Tocopherol and (-Tocotrienol.
  41. Glutathione
  42. M. Asensi, J. Sastre, F.V. Pallardo, J.M. Estrela, and J. Vita, Determination of Oxidized Glutathione in Blood: High Performance Lipid Chromatography.
  43. Carotenoids and Vitamin A
  44. M. Tsuchiya, V.E. Kagan, H.-J. Freisleben, M. Manabe, and L. Packer, Antioxidant Activity of (-Tocopherol, (-Carotene, and Ubiquinol in Membranes: cis-Parinaric Acid-Incorporated Liposomes.
  45. A.R. Sundquist, K. Briviba, and H. Sies, Singlet Oxygen Quenching by Carotenoids.
  46. W. Stahl and H. Sies, Separation of Geometrical Isomers of (-Carotene and Lycopene.
  47. M.A. Livrea and L. Tesoriere, Lipoperoxyl Radical-Scavenging Activity of Vitamin A and Analogs in Homogeneous Solution.
  48. Flavonoids
  49. D.K. Das, Overview of Naturally Occurring Flavonoids: Structure, Chemistry, and High Performance Lipid Chromatography Methods for Separation and Characterization.
  50. W. Bors, C. Michel, and M. Saran, Flavonoid Antioxidants: Rate Constants for Reactions with Oxygen Radicals.
  51. A.E. Hagerman and L.G. Butler, Assay of Condensed Tannins or Flavonoid Oligomers and Related Flavonoids in Plants.
  52. I. Morel, G. Lescoat, P. Cillard, and J. Cillard, Role of Flavonoids andIron Chelation in Antioxidant Action.
  53. J.R.S. Hoult, M.A. Moroney, and M. Paya, Actions of Flavonoids and Coumarins on Lipoxygenase and Cyclooxygenase.
  54. (-Lipoic Acid
  55. Y.J. Suzuki, M. Tsuchiya, and L. Packer, Determination of Structure-Antioxidant Activity Relationships of Dihydrolipoic Acid.
  56. Extracts from Natural Products
  57. L. Marcocci, L. Packer, M.-T. Droy-Lefaix, A. Sekaki, and M. Gardos-Albert, Antioxidant Action of Ginkgo biloba Extract EGb 761.
  58. C. Synthetic Antioxidants: Drugs
  59. H. Sies, Ebselen: A Glutathione Peroxidase Mimic.
  60. P. Moldeus and I.A Cotgreave, N-Acetylcysteine.
  61. M.E. Anderson, E.J. Levy, and A. Meister, Preparation and Use of Glutathione Monoesters.
  62. E.J. Levy, M.E. Anderson, and A. Meister, Preparation and Properties of Glutathione Diethyl Ester and Related Derivatives.
  63. S.J.T. Mao, M.T. Yates, and R.L. Jackson, Antioxidant Activity and Serum Levels of Probucol and Probucol Metabolites.
  64. D.M. Lee, (-Phenyl N-tert-Butylnitrone as Antioxidant for Low Density Lipoproteins.
  65. J.M. Carney and R.A. Floyd, Brain Antioxidant Activity of Spin Traps in Mongolian Gerbils.
  66. L. Marcocci, Y.J. Suzuki, M. Tsuchiya, and L. Packer, Antioxidant Activity of Nitecapone and Its Analog OR-1246: Effect of Structural Modification on Antioxidant Action.
  67. M. Miki, Penicillamine as Antioxidant.
  68. E.D. Hall and J.M. McCall, Antioxidant Action of Lazaroids.
  69. A.M. Miles and M.B. Grisham, Antioxidant Properties of Aminosalicylates.
  70. L. Horakova, H. Sies, and S. Steenken, Antioxidant Action of Stobadine.
  71. M.C. Krishna and A. Samuni, Nitroxides as Antioxidants.
  72. H. Wiseman, Tamoxifen and Estrogens as Membrane Antioxidants: Comparison with Cholesterol.
  73. O.H. Morand, Reactivity of Plasmalogens to Singlet Oxygen and Radicals.
  74. I.T. Mak and W.B. Weglicki, Antioxidant Activity of Calcium Channel Blocking Drugs.
  75. T.G. Gantchev, J.E. van Lier, D.A. Stoyanovsky, J.C. Yalowich, and V.E. Kagan, Interactions of the Phenoxyl Radical of Antitumor Drug, Etoposide, with Reductants in Solution and in Cell and Nuclear Homogenates: Electron Spin Resonance and High-Performance Liquid Chromatography.
  76. Author Index.
  77. Subject Index.


Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
"Incomparably useful."
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
"A series that has established itself as a definitive reference for biochemists."